|
KMID : 0613820210310100929
|
|
Journal of Life Science
2021 Volume.31 No. 10 p.929 ~ p.936
|
|
|
Anti-proliferative Effects and Apoptosis Induced by Chrysin or Emodin in Human Colorectal HCT116 Cells
|
|
Ryu Seung-Min
Kim Yong-Hyun
Lee Eun-Joo
Chung Chung-Wook
Kim Jong-Sik
|
|
|
Abstract
|
|
|
|
In the present study, we screened candidate natural compounds which possess the strong anti-proliferative effects on human colorectal HCT116 cells using the commercial natural product library (Selleckchem, L1400) based on cell viability assay. Human colorectal cancer HCT116 cells were incubated with 50 μM of each compound from the natural product library, and then cell viability was measured by MTT assay. From the first screening, five different kinds of natural products (chrysin, diosmetin, emodin, piperlongumine, and tanshinone I) were selected based on cell viability assay in HCT116 cells and commercial availability. All selected natural products significantly decreased cell viabilities in HCT116 cells, whereas pro-apoptotic protein NAG-1 is strongly induced by chrysin or emodin treatment. Chrysin and emodin decreased cell viability in a dose-dependent manner. Moreover, chrysin and emodin increased the expression of pro-apoptotic NAG-1 protein in a dose- and time- dependent manner. In addition, PARP cleavage induced by chrysin or emodin was recovered in part by the transfection of NAG-1 siRNA indicating that NAG-1 may be one of the genes responsible for apoptosis induced by chrysin or emodin. Overall, our findings may provide basic screening data on natural products which possess anti-proliferative activities and may help to understand the molecular mechanisms of anti-proliferative and pro-apoptotic activities mediated by chrysin and emodin.
|
|
|
KEYWORD
|
|
|
Anti-proliferation, apoptosis, chrysin, emodin, NAG-1
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|